Bio110AntibioticResistanceLabManual2013_

Identityofthekanamycingenepresentinbacterialsamplestak

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Unformatted text preview: e). Set hood with camera on the box so the entire UV light source glass is covered. It is critical that you not expose yourself to the UV light screen once you turn on the unit! 2. Be sure the computer and camera are turned on. Open camera viewing screen and rotate so you can see the image. 3. Be sure slider on camera is turned toward the camera icon. 4. Be sure camera is in Nightscene Mode; if not, use large knob on the top of the camera to select. a. Be sure camera is in “macro” mode. (The flower icon should appear on the lower right of the viewing screen; if not, use the bottom arrow to select). b. If your gel is dim, you may need to switch to manual mode. (0.5‐1.0 sec at F4.0 typically works well. If too dim,use right arrow on function set circle to increase time; if too bright, use the left arrow to decrease time.) 5. For the next steps, you need to work quickly to prevent the lens from fogging. a. Make sure hood is completely covering the blackened glass. Turn on UV light. b. Use camera zoom (toggle surrounding shutter button) to fill frame with gel. Move hood as needed to center. c. Half depress shutter to focus, and then take photo. Turn the UV box off! 6. Move slider on camera back to the left (toward the blue‐boxed arrow). 7. Next, connect the camera USB cable to the computer (if not already connected) and save your image to the desktop. You can then email the image to yourself using Webmail, if time permits. If time is limited, your TA will post your image to your lab’s ANGEL section. 11 8. TURN CAMERA OFF!! After you have photographed your gel, the gel (BUT NOT THE TRAY) should be disposed into the “used gel” disposal bin, and the 1X TAE buffer can be disposed into the “used 1X TAE” disposal bin in the lab room. ASSIGNMENT: Use the information presented by your TA and the lab manual to complete Homework Assignment #6 in which you will explain the purpose of each of the steps of the Flow Diagram (boxes outlined with dashed lines) for answering the research questions that guide the investigation. See syllabus for due dat...
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