Bio110AntibioticResistanceLabManual2013_

Lookatyourgel

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Unformatted text preview: -- + -- -- -- -- + Yellow -- -- + -- -- -- + Red + -- -- + -- -- Green -- + -- -- + -- Pink -- -- + -- -- + ---- While the PCR reaction is running, you will carry out Experiment Part C (counting the number of bacteria colonies growing on plates with and without kanamycin) and prepare a gel for Experiment Part D (identifying genes for kanamycin resistance at each chicken farm). Experiment Part C: Counting Bacterial Cultures Growing on +/‐ Kanamycin Plates Purpose: To determine the frequency of kanamycin resistance in the bacterial population of your chicken farm sample. Create a heading in your notebook: “Experiment Part C: Counting Bacterial Cultures Growing on +/‐ Kanamycin Plates” Procedure Record any intentional or unintentional changes in procedure in your science notebooks 1. Select plates that show individual bacteria colonies (not a smear of bacteria). 2. Look at your plates – if the bacteria have been distributed evenly around the plate, you can divide the plate into 4 equal quadrants and count the number of colonies in a single quadrant. Your total number of colonies can be estimated by multiplying this count by 4. 3. If your bacteria were not distributed evenly around the plate, you will have to count all colonies on the plate. 4. Prepare a table in your science notebook for recording your data. 5. Record the number of colonies on each plate in your lab notebook. 8 6. If you cannot see distinct colonies on your plate – do not count them. Instead, record “lawn” in your lab notebook. NOTE: A lawn will look like a smear – if you can see distinct colonies it is NOT a lawn and you will need to count the number of colonies. 7. Record the number of colonies from your partner’s plates in your lab notebook. Observations Record the number of colonies for each plate on the data table. Indicate “lawn” for plates that have so much growth that individual colonies cannot be distinguished. Experiment Part D: Identifying Genes for Kanamycin Resistance Present in Your Bacterial Sample Purpose: To determine which k...
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This note was uploaded on 02/06/2014 for the course BIO 110 taught by Professor Hass during the Fall '11 term at Pennsylvania State University, University Park.

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