Bio110AntibioticResistanceLabManual2013_

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Unformatted text preview: e. 12 Week 3 Create a heading in your notebook: Patterns in Observations: Analysis of Data I. Frequency of kanamycin resistance in the chicken farm bacterial cultures An important piece of information needed for making recommendations to the CDC about how to handle the bacterial contamination at each chicken farm is the frequency of kanamycin resistance in the original bacterial samples taken from each farm. Refer to the data table you added to your lab notebook that contains your serial dilution data. You will also work with the data from the other groups in your section. Calculate the concentration of bacteria resistant and non‐resistant to kanamycin in the original samples from Lucky Chicken Farm, Mia’s Egg‐cellent Eggs, and Tuckers Cluckers. Calculate the frequency of kanamycin resistance in the bacterial population at each of the chicken farms. Referring to Table 3, use the frequency of kanamycin resistance to determine what level of contamination occurred at the chicken farm where your kanamycin resistance gene originated. Table 3. Recommendation Guidelines for Bacterial Contamination at Egg Producing Facilities Level of Contamination Standard Recommendations Bacterial contamination < 5% Bacterial contamination between 6‐30% Bacterial contamination >31% Divert eggs to pasteurization facility until contamination levels have been <1% for 8 weeks Monitor farm weekly Identify source(s) of contamination Change antibiotic regime Destroy all eggs until levels have been <1% for 8 weeks Identify and destroy infected individuals Monitor farm weekly Identify source(s) of contamination Change antibiotic regime Cull entire population Destroy all eggs Disinfect entire facility Identify source(s) of contamination Submit contamination prevention plan 13 II. Identity of the kanamycin gene present in bacterial samples taken from the chicken farms Referring to the photograph of the gel you created, use the standards for the three possible kanamycin resistance gen...
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