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Unformatted text preview: es to determine the identity of the gene isolated from the chicken farm you are studying. Use the molecular size marker on the photograph to determine the size of the gene. Create a heading in your notebook: Lab Group and Whole Class Discussion of Findings Note: Record notes from this class discussion in your science notebook. This information will be important when you write your research report. In your lab groups discuss: How extensive is the contamination at each farm? What is your evidence? What gene(s) occurred at each chicken farm? What is your evidence? Was more than one chicken farm infected with the same kanamycin resistance gene? What is your evidence? ASSIGNMENT: Your TA will provide information about the writing assignment for this lab. See syllabus for due date. 14 Background Information A. Sterile Technique When working with cultures of bacteria, good technique is essential. We carry bacteria on our own hands, and a wide array of bacterial species are present in the soil and water that we encounter in our daily lives. We do not want to contaminate our laboratory cultures with these visitors. Such contamination would compromise your experimental findings. Your TA will demonstrate the basics of good sterile technique. Keep in mind that there are various levels of sterile technique and as you take other classes and/or gain experience you will likely learn much more about sterile procedures. The following is a general protocol to use when working with bacteria. Before beginning, wash your hands (and/or put on a pair of gloves) and make sure that the area in which you will be handling the bacteria is clean. Disinfect your work area with 70% ethanol from the spray bottle that has been provided and wipe it dry with a clean paper towel. When transferring sterile water or bacteria‐containing cultures, minimize the time when tube caps are off and Petri dish lids are raised. Contaminants tend to fall from above and lids can help reduce the introduction of unwanted bacteria. When inoculating Petr...
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