{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}


Denions and terminologies concerning data and

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: a=on Fragments Sequencing Reads Assembly Con=gs Analysis Our focus for today’s lecture: 1.  Comparison of sequencing plaXorms 2.  Details of sample prepara=on 3.  Defini=ons and terminologies concerning data and sequencing plaXorms History and Background Landmarks in Sequencing Efficiency (bp/person/ year) Year Event 1870 Miescher: Discovers DNA 1940 Avery: Proposes DNA as “Gene=c Material” 1953 Watson & Crick: Double Helix Structure of DNA 1 1965 Holley: transfer RNA from Yeast 1,500 1977 Maxam & Gilbert: "DNA sequencing by chemical degrada=on” Sanger: “DNA sequencing with chain- termina=ng inhibitors” 15,000 1981 Messing and his colleagues developed “shotgun sequencing” method 25,000 1987 ABI markets the first sequencing plaXorm, ABI 370 Landmarks in Sequencing Efficiency (bp/person/year) Year Event 50,000 1990 NIH begins large- scale sequencing bacteria genomes. 200,000 1995 Craig Venture and Hamilton Smith at the Ins=tute for Genomic Research (TIGR) published the first complete genome of a free- living organism in Science. This marks the first use of whole- genome shotgun sequencing, elimina=ng the need for ini=al mapping efforts. 2001 A...
View Full Document

{[ snackBarMessage ]}