Lecture_11_genome_sequencing

Sanger sequencing a g c c g a c t a c t a c t

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: T C G A T C G Sanger Sequencing DNA target sample SHEAR T A T A C G A T C C G A T C G A T C G G 30 Sanger Sequencing DNA polymerase A T A C G Primer Sanger Sequencing DNA polymerase A T A C G Primer Primer A T A C G DNA polymerase Sanger Sequencing A G C Primer DNA polymerase C G A C T A C T A C T A T A C G Sanger Sequencing A G C Primer G DNA polymerase C G A C T A C T A C T A T A C G Sanger Sequencing A G C C G A C T A C T A C T Primer G G A T A C G Sanger Sequencing A G C C G A C T A C T A C T Primer G G C A T A C G Sanger Sequencing A G C C G A C T A C T A C T Primer G G C T G A A T A C G Sanger Sequencing A G C C G A C T A C T A C T Primer Sanger Sequencing A G C Primer C G A C T A C T A C T Con=nue un=l all strands of DNA have undergone this reac=on. If you choose the reagents correctly then you should have all possible A- terminated strands; resul=ng in sequences of varying lengths. Sanger Sequencing Sanger Sequencing In the radioac=ve gel, the longer DNA fragments move to the bopom and the shorter ones move to the top. Aierward the sequence can be read off by going from top...
View Full Document

Ask a homework question - tutors are online