Lecture_11_genome_sequencing

You sck the bit of dna you want to sequence in a liple

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Unformatted text preview: to bopom. Challenges •  Requires a lot of space and >me: you need a place to run the reac=on, and then you need a gel to determine the length of the DNA –  You could only run perhaps a hundred of these reac=ons at any one =m –  There are 3 billion base pairs of DNA in the human genome, meaning about 6 million 500- base pair fragments of DNA •  Nonetheless it was s=ll used to come up with the first copy of the human genome 42 Celera Sequencing (2001) •  •  •  •  •  300 ABI DNA sequencing plaXorms 50 produc=on staff 20,000 square feet of wet lab space 1 million dollars / year for electrical service 10 million dollars in reagents Total cost of human genome: 2.7 Billion dollars Celera Sequencing (2001) •  •  •  •  •  300 ABI DNA sequencing plaXorms 50 produc=on staff 20,000 square feet of wet lab space 1 million dollars / year for electrical service 10 million dollars in reagents Current cost of human genome: < 10,000 $ Second/Next Genera=on Sequencing •  Second genera=on sequencing techniques overcome the restric=ons by finding ways to sequence the DNA without having to move it around. •  You s=ck the bit of DNA you want to sequence in a liple dot, called a cluster, and you do the sequencing there; as a result, you can pack many millions of clusters into one machine. Sequencing a strand of DNA while keeping it held in place is tricky, and requires a lot of cleverness. Illumina Sequencing: Video Steps...
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