Lecture_3_motifs

Atgaccgggatactgatagaagaaaggttgggggcgtacacattagataaacgt

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: is Difficult? atgaccgggatactgatAgAAgAAAGGttGGGggcgtacacattagataaacgtatgaagtacgttagactcggcgccgccg acccctattttttgagcagatttagtgacctggaaaaaaaatttgagtacaaaacttttccgaatacAAtAAAAcGGcGGGa tgagtatccctgggatgacttAAAAtAAtGGaGtGGtgctctcccgatttttgaatatgtaggatcattcgccagggtccga A RNA- seq experiment showed that when gene X is knocked out, 20 other genes are not expressed. How can one gene have such dras0c effects? gctgagaattggatgcAAAAAAAGGGattGtccacgcaatcgcgaaccaacgcggacccaaaggcaagaccgataaaggaga tcccttttgcggtaatgtgccgggaggctggttacgtagggaagccctaacggacttaatAtAAtAAAGGaaGGGcttatag gtcaatcatgttcttgtgaatggatttAAcAAtAAGGGctGGgaccgcttggcgcacccaaattcagtgtgggcgagcgcaa cggttttggcccttgttagaggcccccgtAtAAAcAAGGaGGGccaattatgagagagctaatctatcgcgtgcgtgttcat aacttgagttAAAAAAtAGGGaGccctggggcacatacaagaggagtcttccttatcagttaatgctgtatgacactatgta ttggcccattggctaaaagcccaacttgacaaatggaagatagaatccttgcatActAAAAAGGaGcGGaccgaaagggaag ctggtgagcaacgacagattcttacgtgcattagctcgcttccggggatctaatagcacgaagcttActAAAAAGGaGcGGa AgAAgAAAGGttGGG ..|..|||.|..||| cAAtAAAAcGGcGGG Gene Regula,on A RNA- seq experiment showed that when gene X is knocked out, 20 other genes are not expressed. How can one gene have such dras0c effects? •  Gene X encodes regulatory protein, which we call transcrip0on factor •  The 20 unexpressed genes rely on gene X’s transcrip,on factor to induce transcrip,on. •  A single transcrip,on factor may regulate mul,ple genes. Regulatory Regions •  Every gene contains a regulatory region typically stretching 100- 1000 bp upstream of the transcrip,onal start site •  Located within the regulatory regions are the Transcrip)on Factor Binding Sites (TFBS), also known as mo)fs, specific for a given transcrip,on factor •  Transcrip,on factors influence gene expression by...
View Full Document

Ask a homework question - tutors are online