Lecture2

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Unformatted text preview: our choice of alignment soZware. •  The goal of this lecture is to understand sequence alignment, mo1vate it in the context of assembly, and seeing what soZware is out there. In this lecture… •  Sequence alignment and alignment soZware are used all over bioinforma1cs for different purposes. •  Your results will depend highly on your choice of alignment soZware. •  The goal of this lecture is to understand sequence alignment, mo1vate it in the context of assembly, and seeing what soZware is out there. What is a reference genome? •  A reference genome is a representa1ve example of a species’ set of genes. •  As they are oZen assembled from the sequencing of DNA from a number of donors, reference genomes do not accurately represent the set of genes of any individual. •  BUT the reference is very, very, VERY similar in many loca1ons to the individual. Sample Prepara*on 8 Sample Prepara*on Fragments 9 Sample Prepara*on Fragments Sequencing Next Generation Sequencing (NGS) ACGTAGAATCGACCATG ACGTAGAATACGTAGAA GGGACGTAGAATACGAC Reads 10 Sample...
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This note was uploaded on 02/10/2014 for the course CS 680 taught by Professor Staff during the Fall '08 term at Colorado State.

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