2009Spring Test 1

Com youare studying enzymes b b oth of w hich act

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Unformatted text preview: A s u b s t r a t X ( X - e n z - A Y a n dX - e n z - B Z ) . Y o um e a s u r e e ) ) e t .! ED4 11. COM - PR EM the r ate o f the r eactionX ) Y b y enzymeA a nd enzymeI against v a r y i n g m o u n t s f X .T h ee n z y m e o n c e n t r a t i o n s e e x a c t l yh e a o c ar t s a m ei n e a c he x p e r i n r e nIth e r e 5 u l t a r e p l o t t e d n t h e f i g u r e o t h e . s i t r i g h t . n s w e t h e n e x t3 q u e s t i o n s . A r .9-I 0 1.0 2.5 q t5 Conc6 r rtion of X ( pMt 10 11. COM - PR EM X 33. . W hichenzymehasa g reatera ffinityf or t he substrate ? B a) e nzyme A b)enzyme c ) n e i t h e rt,h e yh a v et h e s a m e d) n one of theseis correct MED 411 .CO MPRE M ED4 A 3 4 . M o l e c u l e c a nb i n dt o e n z y m e a n dw h e n i t d o e si t , i t c h a n B ets e Z h r a t eo f r e a c t i o n f e n z y m e s o t h a t i t h a st h e s a m e a xa ' s n u n b o u n d n z y m e a t h i g h[ X ] ,b u t t h e K mo f t h e Z b o u n d o A V a e A What is l ikelyto be t|aBpening. enzyme i s larger. A in o a)irreversiblehibitionf A byZ bj c o m p e t i t i v i n h i b i t i o n f A b y Z e i o c) n on c ompetitive nhibitiono fA by Z d) p roteolytic leveage enzymeA c of e ) n one o f thesei s correct 411 .CO MPRE i 3 5 ., W h i c ho f t h e f o l l o w i n gs c o r r e c c o n c e r n i ne n z y m e A a n d B ? t s g ' a ) V m a x A< V m a x B .b ) . K r F A - - t - K m B c ) V m a x A= K m B B d )V m a x = K m A e) none of these MPRE MED 411 .CO MPRE MED o f 3 6 . A m o l e c u l e f t h e m o t o rp r o t e i n i n n e b a g ( s e e i g ) ,s u p p l i e d i t h A T P , W o w i s m o v i n g l o n g m i c r o t u b u lie t h e d i r e c t i o n h o w n W h a t i s l i k e l y o h a p p e n a a n s . t w if y ou s uddenly ashawaya ll the ATPfrom the systemby addinga n enzymethat h ydrolyzes ATP? o w a ) n o c h a n g eW i n n e B a bw i l l c o n t i n u eo m o v ef r o m A t o B , t W innebago ill move b ackwardsrom B toward f _b) l i k e l y t a l lo n t h e m i c r o t u b u l e c ) W i n n e b a gw i l l c o n t i n u eo m o v ef r o m A t o B b u t a t a s l o w e r a t e o t d ) W i n n e b a gw i l l o s e ) n o n eo f t h e s ei s l i k e l v PRE MED 411 .CO m o 3 7 . A c o m m o n e a n s f p r o v i d i n g n e r g yo a n e n e r g e t i c a luy f a v...
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This note was uploaded on 02/08/2014 for the course BIL 255 taught by Professor Skromme during the Fall '08 term at University of Miami.

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