PRACTICAL REPORTMarkFaculty:ScienceProgram:Agricultural Science / Food ScienceCourse Code & Title:UDAF1104INTRODUCTORYMICROBIOLOGY__________________Trimester:January 2021Exp. :Practical 2: Aseptic Technique and Transfer ofMicroorganisms__________________________________Practical Group:P1_____________________Date of Submission:16thFebruary 2021_______________________Instructor:Dr.Tan JiDeclaration:I/ We certify that this report is our own work, where material and reference used fromother sources have been properly acknowledged.GroupmembersNameID NumberSignatureAssessmentcriteriaMarksallocated(%)Cover page5Introduction10Materials andMethods10Results20Discussion20Conclusion10References10General formattingand language15Total100
1LIM CHEA19ADB033202LIM XIN WEI19ADB033993TAN CHUN HAU19ADB0293045
Introduction:With common sense, our environment comes with various types of mixedmicroorganisms. The study of microbiology requires us to study the microbial world throughvarious species of individual microorganisms. Therefore, special techniques are needed forseparating microorganisms into their individual pure colonies for us to study. With the help ofthese techniques, the study of the uses, habits and treatments for every microorganisms canbe more effective. In the modern days, these techniques are called aseptic technique andtechnique of transferring microorganisms.Aseptic technique is very crucial and used for minimizing the contamination, and alsoprevents the person from getting infection when transferring microorganisms. Aseptictechnique can come in many ways from applying alcohol to disinfect, burning the apparatusto sterile, all possible ways of killing and stopping the microorganisms are included as aseptictechnique. As our laboratory studies come from pure culture, constantly sterile anddisinfecting apparatuses like heating the inoculation loops and needles until red-hot areneeded to make sure no contamination to both, the pure culture and during its transfer.Technique of transferring microorganisms can be classified into broth culture, agarslope and pure culture techniques. Before applying the following techniques, the culturemedium also known as agar needs to be prepared. According to Laboratory News (2005), theagar is a compound name polysaccharide which is derived from red seaweeds, it has the bestin both physical and chemical properties to help improve the study of microbes anddiscovered by Walther Hesse (1846-11), by hearing his wife Fanny Hesse (1850-34) advise toreplace gelatin with agar. Culture medium can come with 2 physical states liquid and solid,normally liquid medium is call broth and solid medium is call agar, agar act as solidifyingagent to make the culture medium into solid state in room temperature and broth does not hasany solidifying agent so the culture medium will remain liquid in room temperature.