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2 octopine ti plasmid agrobacterium tumefaciens 1

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Unformatted text preview: plasmid carries two genes that direct production of: 1. 2. Octopine Ti plasmid Agrobacterium tumefaciens 1. Agrobacterium transfers the Ti plasmid to the plant by conjugation. 2. Ti plasmid is inserted into a plant chromosome. 3. Plant expresses Ti genes: • rapid plant growth (tumor) • plant makes octopine 4. Agrobacterium feeds on the octopines!!! Agrobacterium tumefaciens What if a genetic engineer removed the growth hormone gene and the octopine gene from the Ti plasmid… And replaced them with a gene that codes for Vitamin A synthesis? Golden Rice Who eats the Vitamin A? Humans 250,000 - 500, 000 children Transduction TRANSDUCTION: transfer of genetic material between two bacteria… PHAGE: Phage replication Makes many copies of phage chromosome and lots of phage proteins Fig 7.34 Phage replication Normal infectious phage TRANSDUCING PARTICLE Transducing particles Transducing particle transfers _____________DNA NOT phage DNA Theoretically, any piece of the chopped up host chromosome could be transferred...
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