Lecture+5+-+Genetics+Outline

Can bind each sigma recognizes a specific promoter

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Unformatted text preview: RNA polymerase can bind Each sigma recognizes a specific promoter sequence. Determines which messages get sent “Core” plus “Sigma” = RNA Polymerase Promoters Promoters are DNA sequences that indicate where to start transcription (start the message) “-35 box” and “-10 box” are promoters σ sigma 3’ DNA 5’ RNA Polymerase Fig 7.9 Promoters There are lots of different promoters that are recognized by σ Some promoters are strong, others are weak Strong promoters call over lots of RNA Polymerases. So the microorganism will create… lots of messages lots of messages= lots of proteins? Transcription RNA polymerase unwinds a small piece of DNA into single strands RNA nucleotides float in and base pair with the complementary DNA σ 5’ 3’ -35 5'----------------------------------- -10 3’ -------3' 5’ RNA Polymerase Template strand (DNA) RNA polymerase polymerizes RNA! RNA is central to protein synthesis rRNA (ribosomal): forms structure of the ribosome, recognizes messages, assembles proteins tRNA (transfer): activates amino acids and reads the mRNA message 30S 50S 5’ 3’ mRNA (messenger): carries the message from the DNA (gene) Protein synthesis (Translation) N’ M A W L L Q A K D K G A A L G ‘C A proteins shape/function is determined by its primary sequence of amino acids There are 20 different amino acids This unique primary structure must be assembled in the correct order during translation…or else Ribosomes 30S Protein + rRNA 50S Prokaryote 7...
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This note was uploaded on 02/10/2014 for the course CHEM 341 taught by Professor Skagg during the Spring '11 term at Indiana.

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