Molecular biology techniques

Aim sourceofdna steps examplesofapplications

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Unformatted text preview: ein cDNA Clone cDNA in vector with inducible bacterial promoter (Usually the a tag sequence is added that allow purification of the recombinant protein by affinity purification) Transformation Large scale growth Induction of transcription ‐> protein production Protein isolation Production of antigen to raise antibodies Biochemical studies Production of enzymes (for biochemical research, for medical applications, for biotechnology) 6. Aim: Source of DNA: Steps: Examples of applications: Construction of mutants U...
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This note was uploaded on 02/13/2014 for the course BIOL 200 taught by Professor Unknown during the Fall '97 term at The University of British Columbia.

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