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Clone cDNA in vector with inducible bacterial promoter (Usually the a tag sequence is added that allow purification of the recombinant protein by affinity purification) Transformation Large scale growth Induction of transcription ‐> protein production Protein isolation Production of antigen to raise antibodies
Biochemical studies Production of enzymes (for biochemical research, for medical applications, for biotechnology) 6. Aim: Source of DNA: Steps: Examples of applications: Construction of mutants
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