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Unformatted text preview: se a cDNA to create new mutants
Clones cDNA in appropriate vector (e.g. with useful restriction sites)
Delete pieces (cut and religate) or create “point mutations” (single or few basepairs). “Structure‐function analysis” = determine which region of the protein or which specific amino‐acid is responsible (or required) for the function of the protein (e.g. enzymatic activity, binding to another protein, binding to a substrate, selectivity for ions in the case of a channel, subcellu...
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