Molecular biology techniques

Determinetheeffectonfunctionofspecificmutationsdetecte

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Unformatted text preview: se a cDNA to create new mutants cDNA Clones cDNA in appropriate vector (e.g. with useful restriction sites) Delete pieces (cut and religate) or create “point mutations” (single or few basepairs). “Structure‐function analysis” = determine which region of the protein or which specific amino‐acid is responsible (or required) for the function of the protein (e.g. enzymatic activity, binding to another protein, binding to a substrate, selectivity for ions in the case of a channel, subcellu...
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