Molecular biology techniques

Bcutthevectorcontainingthecdnaandinsertbyligationthe

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Unformatted text preview: lar localization, and so on…). Determine the effect on function of specific mutations detected for instance in a Drosophila mutant or in a genetic disease 7. Aim: Source of DNA: Steps: Examples of applications: Construction of DNA coding for “fusion proteins” Create a fusion between two coding sequences cDNA a) Ligate full length cDNA or fragment/mutant (depending on experiment) in a vector already containing another coding sequence, either 5’ or 3’ of the inserted fragment (note that the two coding sequences must be “i...
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This note was uploaded on 02/13/2014 for the course BIOL 200 taught by Professor Unknown during the Fall '97 term at The University of British Columbia.

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