Molecular biology techniques


Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: lar localization, and so on…). Determine the effect on function of specific mutations detected for instance in a Drosophila mutant or in a genetic disease 7. Aim: Source of DNA: Steps: Examples of applications: Construction of DNA coding for “fusion proteins” Create a fusion between two coding sequences cDNA a) Ligate full length cDNA or fragment/mutant (depending on experiment) in a vector already containing another coding sequence, either 5’ or 3’ of the inserted fragment (note that the two coding sequences must be “i...
View Full Document

This note was uploaded on 02/13/2014 for the course BIOL 200 taught by Professor Unknown during the Fall '97 term at The University of British Columbia.

Ask a homework question - tutors are online