Chris HermanAssignment 2In the peer-reviewed article, Molecular Phylogenetics of the New World Bat Genus Phyllostomus based on Cytochrome B DNA Sequence Variation, Ronald A. Van Den Bussche and Robert J. Baker research the phylogenetic relationship of the New World bat family, Phyllostomidae and other higher level taxonomic levels. In addition to these relationships, Bussche and Baker study charactersets of karyology, skeletal morphology, uterine histomorphology, immunology, allozymes and rDNA restriction-site data. To study these relationships Bussche and Baker use cladistic analyses of Phyllostomus to show how its phylogenetic characteristics are primitive. This paper shows phylogenetic relationships between Phyllostomus and Phylloderma by creating a phylogenetic tree based on immunological distances between the two families and subfamilies. One major limitation Bussche and Baker encountered when studying these relationships was the fact that there is not a lot of research done on these families so Bussche and Baker were forced to come up with their own phylogenetic tree and creating clades based on their own findings. However, they mention how new advances in DNA studies such as tests like Polymerase Chain reaction, or PCR, they were able to study the genomes of these families making comparisons much easier. Throughout the article, Bussche and Baker show comparisons between the taxa by showing the nucleotide variations between each individual. These comparisons show where specific phylogenetic relationships come from.Citation:Ronald A. Van Den Bussche, Robert J. Baker. Moledular Phylogenetics of the New World Bat Genus Phyllostomus Based on Cytochrome B DNA Sequence Variation. August 1993. 74. 793-802.