Lee metbolic eng synthetic bio BME 162 GuestLecture Apr2_2012

G cancer 4212 bme 162 guest lecture 3 prospects for

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Unformatted text preview: cialty chemicals (e.g. amino acids, bio ­polymers) •  Biomass derived carbon fuels •  Produce chemicals new to the host organism –  Drug discovery (e.g. ‘natural products’ as precursors for an0bio0cs, an0oxidants, and cancer therapeu0cs) •  Biomedical applica0ons –  Elucida0on of disease mechanisms –  Iden0fica0on of targets for metabolic diseases, e.g. cancer 4/2/12 BME 162 Guest Lecture 3 Prospects for Biofuels 2000 - 2007 Projections for 2030 4/2/12 BME 162 Guest Lecture 4 Tradi0onal route for alcohol fuels is fermenta0on Note: Not all of these pathway will be present in a single organism sugars + 2H lactate PYR ATP + pyruvate - CO2 + CO2 OAA + CoA ADP + 4H CO2 succinate - CO2 propionate - CoA H2 - H 2O + 2 H - CO2 CO2 Acetoin H2 acetyl-CoA formate + 2H malate Acetolactate ethanol 2,3-butanediol + acetyl-CoA, - CoA - CoA acetic acid + 2H acetoacetyl-CoA + 4H butyryl-CoA - CO2 - CoA-SH acetone - CoA + 2H butyric acid isopropanol + 4H - CoA butanol Fermentation in Clostridium* acetobutylicum Thl/PhaA: acetyl-CoA acetyltransferase CtfAB/AtoAD: acetoacetyl-CoA transferase Adc: acetoacetate decarboxylase Adh: alcohol dehydrogenase Hbd: 3-hydroxybutyryl-CoA dehydrogenase Crt: crotonase Bcd-EtfAB/Ter: butyryl-CoA dehydrogenase AdhE2: aldehyde/alcohol dehydrogenase 4/2/12 BME 162 Guest Lecture 5 Direct synthesis from amino acids is also possible Atsumi et al. Nature. 2008;451(7174):86-9. 4/2/12 BME 162 Guest Lecture 6 Alcohols from Protein Biomass Huo et al. Nat Biotechnol. 2011;29(4):346-51. 4/2/12 BME 162 Guest Lecture 7 The direct synthesis pathway could also be used for carbon capture Integration of synthesis steps and enhanced Rubisco in a cyanobacterium (Synechococcus elongatus) Productivity comparisons Atsumi et al. Nat Biotechnol. 2009;27(12):1177-80. 4/2/12 BME 162 Guest Lecture 8 Natural Products •  Natural occurrence – ...
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