{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Lee metbolic eng synthetic bio BME 162 GuestLecture Apr2_2012

G cancer 4212 bme 162 guest lecture 3 prospects for

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: cialty chemicals (e.g. amino acids, bio ­polymers) •  Biomass derived carbon fuels •  Produce chemicals new to the host organism –  Drug discovery (e.g. ‘natural products’ as precursors for an0bio0cs, an0oxidants, and cancer therapeu0cs) •  Biomedical applica0ons –  Elucida0on of disease mechanisms –  Iden0fica0on of targets for metabolic diseases, e.g. cancer 4/2/12 BME 162 Guest Lecture 3 Prospects for Biofuels 2000 - 2007 Projections for 2030 4/2/12 BME 162 Guest Lecture 4 Tradi0onal route for alcohol fuels is fermenta0on Note: Not all of these pathway will be present in a single organism sugars + 2H lactate PYR ATP + pyruvate - CO2 + CO2 OAA + CoA ADP + 4H CO2 succinate - CO2 propionate - CoA H2 - H 2O + 2 H - CO2 CO2 Acetoin H2 acetyl-CoA formate + 2H malate Acetolactate ethanol 2,3-butanediol + acetyl-CoA, - CoA - CoA acetic acid + 2H acetoacetyl-CoA + 4H butyryl-CoA - CO2 - CoA-SH acetone - CoA + 2H butyric acid isopropanol + 4H - CoA butanol Fermentation in Clostridium* acetobutylicum Thl/PhaA: acetyl-CoA acetyltransferase CtfAB/AtoAD: acetoacetyl-CoA transferase Adc: acetoacetate decarboxylase Adh: alcohol dehydrogenase Hbd: 3-hydroxybutyryl-CoA dehydrogenase Crt: crotonase Bcd-EtfAB/Ter: butyryl-CoA dehydrogenase AdhE2: aldehyde/alcohol dehydrogenase 4/2/12 BME 162 Guest Lecture 5 Direct synthesis from amino acids is also possible Atsumi et al. Nature. 2008;451(7174):86-9. 4/2/12 BME 162 Guest Lecture 6 Alcohols from Protein Biomass Huo et al. Nat Biotechnol. 2011;29(4):346-51. 4/2/12 BME 162 Guest Lecture 7 The direct synthesis pathway could also be used for carbon capture Integration of synthesis steps and enhanced Rubisco in a cyanobacterium (Synechococcus elongatus) Productivity comparisons Atsumi et al. Nat Biotechnol. 2009;27(12):1177-80. 4/2/12 BME 162 Guest Lecture 8 Natural Products •  Natural occurrence – ...
View Full Document

{[ snackBarMessage ]}

Ask a homework question - tutors are online