Exercise 3 Introduction to Basic Light Microscopy
Tools of Observation • Initial studies of natural phenomena made with unaided senses, sight, smell, hearing, touch. • However, our senses are limited: • We can only detect a narrow section of the electromagnetic spectrum (between 750 nm and 400 nm) • Resolution : For unaided human eye, the closest that two objects (or edges) can be together and still distinguished as separate entities ~0.1 mm • Tools of observation allow us to extend our senses make us aware of natural phenomena invisible to our senses.
Objectives of Exercise 2 • Learn how to use the compound brightfield microscope. • Obtain Kohler Illumination. • Make microscopic observation of a permanent dry mount. • Prepare a wet mount and make microscopic observations. • Make microscopic measurements using an ocular reticle. • Calibrate the ocular reticle.
* Optical Microscopes • A microscope is an instrument for viewing objects that are too small to be seen by the unaided eye. • An optical microscope (also called a light microscope) uses light to project a magnified view of the object. Some examples of light microscopes