{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

04BIS10110152013RecombinLect4

This is typically done using a test cross to an f1

Info icon This preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: rgan or 1 cM) ­ (Remember, 1 crossover produces 2 crossover gametes so recombinant (Remember, progeny must be added together when calculating % recombination as shown below) In Morgan’’s 2-factor cross, the genetic distance between s shown the pr and vg loci can be determined by calculating the frequency of pr vg recombinant progeny. recombinant 270 + 270 Total Recombinant Progeny Total Progeny X 100 5849 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez X 100 = 9.2% 26 Constructing Genetic Maps ­ A genetic map is a genetic means of displaying the number of genes, their number order along the order chromosome and their relative distances from relative each other. This is typically done using a test cross to an F1 test individual carrying different alleles (heterozygous) for at least two genes or at two loci. BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 27 3 Approaches to Constructing Genetic Maps ­ ­ ­ Approach 1. Determining gene order by performing multiple, Determining two factor crosses using overlapping loci. Gene order will be revealed by consistent map distances. revealed Approach 2. Perform a 3-factor test cross (F1 x tester) and determine genetic distances first. As above, gene order will be revealed by consistent map distances between the three loci. This approach can be performed without rare double crossover progeny. Approach 3. Perform a 3-factor cross (F1 x tester) and determine the gene order first. This is done by comparing parental phenotypes to double crossover phenotypes. From the gene order, recombinant progeny produced by recombination between loci can be calculated and these calculations will take into account double crossover progeny. If double crossover progeny are not accounted for, the map distances will not add up properly. distances BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ...
View Full Document

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern