04BIS10110152013RecombinLect4

P o6373221401000 x 10014 ii o s11096222101000

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: ouble Crossovers BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 33 Tomato Trihybrid Cross with Double Crossovers Progeny Phenotypes + + o + o + o o + + + p + p p p + s s s + + + s 110 348 2 63 73 2 306 96 96 1000 data sort + o + o o + o + + p + p + p + p Compare parental and Compare double crossover progeny s 348 ++s + 306 op+ + 110 s 96 o+s + 73 +p+ s 63 s2 +2 1000 1000 New Gene Order ++s I II II po+ BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 34 Tomato Trihybrid Cross with Double Crossovers Progeny Phenotypes New Gene Order + + s 348 ++s o p + 306 I II II + + + 110 po+ o p s 96 o + + 73 + p s 63 Sum all recombinants resulting from o+s 2 crossovers in domains I, II and I & II I, crossovers +p+ 2 1000 1000 I. p-o:63+73+2+2+=140/1000 x 100=14% II. o-s:110+96+2+2=210/1000 x 100=21% I and II. p-s:73+63+110+96+(4 x 2*) =350/1000 x 100=35% *Without these double crossovers multiplied by 2, the map distances between p and s would be 34.6, not the sum of 14+21 or 35. BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 35 Tomato Trihybrid Cross with Double Crossovers New Gene Order ++s I II II po+ I. p-o:63+73+2+2+=140/1000 x 100=14% II. o-s:110+96+2+2=210/1000 x 100=21% I+II. p-s:73+63+110+96+(4 x2)=350/1000 x 100=35% 35 p A. 14 o 21 35 B. s s o 14 p 21 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 36 Drosophilia Trihybrid Cross with double crossovers: 2 F2 Phenotype Male cut wing + + ct F2 Genotype Female + + ct cv v ct No. of Progeny 759 1525 crossveinless wing vermillion eye vermillion eye cv v + cv v + cv v ct 766 +v+ +v+ cv v ct 73 153 crossveinless cut wing cv + ct cv + ct cv v ct 80 vermillion eye cut wing + v ct + v ct cv v ct 140 crossveinless wing cv + + cv + + cv v...
View Full Document

This note was uploaded on 02/12/2014 for the course BIS 101 taught by Professor Simonchan during the Spring '08 term at UC Davis.

Ask a homework question - tutors are online