09BIS1012013TransciptLect9-1

Lrodriguez 2013

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Unformatted text preview: expressed as: α 2ββ 'σ expressed (480kD). (480kD). Without the σ subunit, RNAP Without is referred to as "core is polymerase" and is capable polymerase and of elongation of RNA synthesis and nonspecific transcription of dsDNA. β’ β α α β’ β’ α α σ RNAP holoenzyme σ BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 11 Sense Transcription Sigma is needed for recognize the promoter DNA sequence Sigma and position the RNAP holoenzyme so that the β’ subunit can bind the “template” (transcribed) strand as opposed to the (transcribed) “non-template” (non-transcribed) strand. (non-transcribed) The non-template DNA strand is also called the “RNA-like The RNA-like strand” because it is identical in sequence to the newly made strand because RNA molecule. RNA BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 12 Sense and Antisense Strands 5’­A G C C T A T T G G G A T C T A T T A A T C C C­3 ’ non-template, nontranscribed or sense strand template, transcribed or antisense strand 3’­T C G G A T A A C C C T A G A T A A T T A G G G­5 ’ 5’­A G C C T A T T G G G A T C T A T T A A T C C C­3 ’ sense strand RNA 5’A U U G G G A U C U A U U A A U C C 3’­T C G G A T A A C C C T A G A T A A T T A G G G­5 ’ 5’­A G C C T A T T G G G A T C T A T T A A T C C C­3 ’ C C C U A G A U A A U U A G G G­5 ’ antisense strand RNA 5’A U U G G G A U C U A U U A A U C C 3’­T C G G A T A A C C C T A G A T A A T T A G G G­5 ’ BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 13 Transcription Initiation Because the subunits of RNAP are positively charged (rich in arginine and lysine), it has a natural affinity for negatively c...
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