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nucleotides that appear that in the promoter sequence, less than (lower
case) and more than (upper case) 50% of the time. Subscript numbers
represent percentages of occurrence while numbers above the sequence
refer to conserved regions identified by Pribnow. Mutation analysis has
shown that these conserved regions are required for the promoter
function. Upward arrows in the second row refer to enhanced
transcription, down arrows refer to mutations that decrease transcription.
Mutations that change the sequence toward consensus increase
ctT82T84G78A65C54a45-----15 to 18 bp--------T80A95t45A60a50T96--4 to 9 bp---cat-(iq)
T C T (UV5)
t c T T G A C a -------(15 to 18 bp) --------T A t A a T -----------c a t-----A A T AG
A BIS101001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013
BIS101001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 21 Example of 3 Common Bacterial Promoters 35 10 +1
3'GGTCCGAAATGTGAAATACGAAGGCCGAGCATATTACACACCTTAACACTCGCCTATTGTTAAAGTG SD site Met
TGTCCTTTGTCTTAAGATAC5’ E. coli lac UV5 35 10 +1
3'CTTAAGAGTACAAACTGTCGAATAGTAGCTATTCGAAATTACGCCATCAAATAGTGTCAATTTAACGA SD site Met
TTGCGTCAGTCCGTGGCACATAC5’ pBR322 Resistance 35 10 +1
SD site Met
AGTTATTATAACTTTTTCTTCTCATAC5' pBR322/pUC19 Ampicillin Resistance B...
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This note was uploaded on 02/12/2014 for the course BIS 101 taught by Professor Simonchan during the Spring '08 term at UC Davis.
- Spring '08