09BIS1012013TransciptLect9-1

Lrodriguez t2rna 31 29 20 18 7 evidence for rna as an

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Unformatted text preview: E coli RNA T2 DNA 25% 25% 25% 25% 23% 23% 31% 23% 32% 32% 18% 18% BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez T2RNA 31% 29% 20% 18% 7 Evidence for RNA as an Intermediate Messenger David Prescott’’s pulse/chase experiments using 3H-cytidine s H-cytidine and auto-radiography showed that RNA was made in the nucleus and transported to the cytoplasm. nucleus BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 8 Rules & Requirements for specific gene transcription Double-stranded DNA (dsDNA) The 4 ribonucleotide triphosphates ATP, CTP, GTP and The UTP UTP A regulatory DNA sequence called a “promoter” regulatory RNAP holoenzyme (core + σ ) RNAP holoenzyme 5’’-3’ de novo polymerization -3’ de BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 9 Structure of Bacterial RNAP Holoenzyme RNAP holoenzyme is a multi-component enzyme consisting RNAP holoenzyme of at least 5 subunits. subunits. The structure of the holoenzyme can be expressed as: α 2ββ 'σ (480kD). Without the σ subunit, RNAP is referred to as "core Without core polymerase" and is capable of elongation of RNA synthesis polymerase and and nonspecific transcription of DNA. BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 10 RNAP Holoenzyme RNAP holoenzyme is a RNAP holoenzyme multi-component enzyme consisting of at least 5 subunits. subunits The structure of the The holoenzyme can be...
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This note was uploaded on 02/12/2014 for the course BIS 101 taught by Professor Simonchan during the Spring '08 term at UC Davis.

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