09BIS1012013TransciptLect9-1

The in eukaryotes the initial rna transcript

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Unformatted text preview: nation of Special the transcription process. the In eukaryotes, the initial RNA transcript (heterogeneous nuclear In RNA — hnRNA) is processed in several way to generate the final mRNA. mRNA. Many eukaryotic genes contain intervening DNA sequences called Many “introns” that must be spliced out of the corresponding hnRNA. “introns” HnRNA can be spliced in several ways. One method requires the HnRNA action of an RNA-containing enzyme called a splicosome. action BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 3 Review of Central Dogma of Molec. Biol. Transcription-Splicing-Translation BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 4 Transcription/Replication Transcription and DNA replication are linked genetically and Transcription biochemically. Genes for RNA polymerase are mixed in with genes for Genes replication and translation and co-transcribed (a “regulon”). 66’ rpsU dnaG rpsU (S21) primase (S21) rpoD 67’ σ subunit 70kD 70kD 72’ rpoA rpsD rpsK rpsM 71’ rpoA rps rpsK α subunit subunit 40kD 40kD 84’ 88’ rplJ rplL rpoB rpoC 89’ rplJ rplL rpoB β & β ’ subunits 151kD 155kD 151kD E. coli origin of DNA replication - Ori C BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 5 Transcription Because DNA does not participate directly in the synthesis of the gene Because product (usually a protein), an intermediate information-containing molecule is needed. This molecule is called messenger RNA (mRNA) and is synt...
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This note was uploaded on 02/12/2014 for the course BIS 101 taught by Professor Simonchan during the Spring '08 term at UC Davis.

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