10BIS1012013EuKTranscLect10

Aauaaa the cleavage stimulation factor cstf an rnathe

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Unformatted text preview: bases) complementary (3'UUAUU5') to the poly A site. This polyA tail may prevent 3' degradation and increase the This polyA half-life of the mRNA. BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 22 Steps in Tailing of mRNA ­A specificity factor, Cleavage and Polyadenylation specificity Specificity Factor (CPSF), consists of three subunits, Specificity each of which is an RNA-binding protein. Each of the individual subunits will bind RNA non-specifically. However, collectively, they recognize and bind specifically to the AAUAAA polyadenylation sequence. AAUAAA ­The Cleavage Stimulation Factor (CStF) an RNAThe Cleavage binding protein, is required to stabilize the CPSF and binding endonuclease. This endonuclease consists of two endonuclease components -- CFI and CFII. It cleaves the RNA. CFI CFII ­Once these proteins have assembled, the mRNA is Once cleaved 10-35 bases downstream of the AAUAAA AAUAAA recognition sequence by the endonucleas...
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This note was uploaded on 02/12/2014 for the course BIS 101 taught by Professor Simonchan during the Spring '08 term at UC Davis.

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