10BIS1012013EuKTranscLect10

Aauaaa the cleavage stimulation factor cstf an rnathe

Info icon This preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: bases) complementary (3'UUAUU5') to the poly A site. This polyA tail may prevent 3' degradation and increase the This polyA half-life of the mRNA. BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 22 Steps in Tailing of mRNA ­A specificity factor, Cleavage and Polyadenylation specificity Specificity Factor (CPSF), consists of three subunits, Specificity each of which is an RNA-binding protein. Each of the individual subunits will bind RNA non-specifically. However, collectively, they recognize and bind specifically to the AAUAAA polyadenylation sequence. AAUAAA ­The Cleavage Stimulation Factor (CStF) an RNAThe Cleavage binding protein, is required to stabilize the CPSF and binding endonuclease. This endonuclease consists of two endonuclease components -- CFI and CFII. It cleaves the RNA. CFI CFII ­Once these proteins have assembled, the mRNA is Once cleaved 10-35 bases downstream of the AAUAAA AAUAAA recognition sequence by the endonucleas...
View Full Document

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern