10BIS1012013EuKTranscLect10

Carboxy terminal domain must be phosphorylated to

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Unformatted text preview: ssed in the nucleus. processed RNAPII, ~520 kDal, 10 subunits, very sensitive to α-amanitin, transcribes -amanitin, hnRNA (pre mRNA). Carboxy-terminal domain must be phosphorylated to activate transcription RNAPIII, ~700 kDal, 12 subunits, RNAPIII, moderately sensitive to α-amanitin, -amanitin, transcribes tRNA and 5S rRNA genes. transcribes BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 5 The Eukaryotic Promoter ­ As shown below, the TATA box is located around -25 (instead of the Pribnow box at -10) As while the CAAT box is typically found between -60 to -80, (instead of at -35). The consensus eukaryotic promoter is shown below. consensus -80 to -60 -25 +1 -80 (enhancers)--GGCTCAATCT---T82% A97% T93% A85% A63% A85% A50%------c-A-t-(enhancers)--GGCTCAATCT---T82% 97% T37% T33% BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 6 E...
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