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BIS101001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 11 Eukaryotic Transcription Complex
consists of many
factors Factors include: Activators Repressors Coactivators Basal factors Non-coding RNAs BIS101001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013
BIS101001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 12 Formation of Active Eukaryotic Initiation Complex
The diagram at the right shows the
complexes formed on the TATA box by
the transcription factors and RNA
polymerase II (RNAPII)
(A) The TBP complex’s with TFIID to
bind to the TATA box.
(B) TFIID is stabilized by TFIIA and TFIIB
and binds to the TATA box.
(C) TFIIE and TFIIH join the complex on
the TATA box with RNAPII with TFIIF
and other factors.
(D) RNAPII is positioned by TFIIB, and
its carboxy-terminal domain (CTD) is
bound by TFIID.
(E) The CTD is phosphorylated by TFIIH
and is released by TFIID. The RNAPII is
now competent to tr...
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