10BIS1012013EuKTranscLect10

Lrodriguez 2013

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Unformatted text preview: g 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 26 Intron Splicing of Eukaryotic mRNA ­ The presence of intervening DNA sequences (introns) iin eukaryotic genes n was discovered almost simultaneously in the mid 70's by Philip Leder (hemoglobin) and Bert O'Malley (ovalbumin). (hemoglobin) BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 27 EM of Introns and R loops Introns can be visualized by electronmicroscopy (EM) by first denaturing ovalbumin genomic DNA and then hybridizing complementary mRNA to it. This creates R-Loops. R-Loops A C D F G B E BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 28 Splicing Introns from Eukaryotic mRNA Splicing ­ The discovery of introns was aided by two technologies; RNA (northern) blotting The and electron microscopy of RNA/DNA hybrids. The introns of...
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This note was uploaded on 02/12/2014 for the course BIS 101 taught by Professor Simonchan during the Spring '08 term at UC Davis.

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