10BIS1012013EuKTranscLect10

Lrodriguez 38 alternate splicing of mrnas in the

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Unformatted text preview: e Expression, R.L. Rodriguez 35 Intron Splicing is ordered and sequential ­ Introns are spliced in a specific order and sequence. They are not spliced Introns from left to right. Splicing order probably depends on the homology between the splice junction sequences and the snRNAs. between BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 36 Splicing Summary BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 37 The X-Linked Human Dystrophin Gene With more than 80 introns, the human dystrophin gene (Xp21) is an extreme example of splicing. The primary transcript (hnRNA) is >2.5Mb while the mRNA is 14,000 bases. Mistakes in intron splicing are associated with Duchenne (DMD) and Becker (BMD) muscular dystrophy. Duchenne BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 38 Alternate Splicing of mRNAs ­ ­ ­ ­ In the example at the right, In membrane-bound and secretory antibodies are made from the same heavy-chain gene by alternate splicing. In this case, exon 6 contains a “splice donor” (5’GU) in the middle of the exon. (5’GU) Splicing that removes the 6b part Splicing of exon 6 produces the membrane bound antibody. membrane Splicing...
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