10BIS1012013EuKTranscLect10

This is necessary if the mrna is to be colinear with

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Unformatted text preview: nuclear genes are unique in that they share short, highly conserved sequences (GU/AG) in the unique GU/AG) junctions between the exon and intron. Splicing is said to obey the GU/AG rule. GU/AG Intron 1 5’ Exon1 3’ A64G73/G100U100A62A68G84U63 (Py Stretch)-18-40nt-A100G100/N Exon2 Branch point Py80 N Py80 Py87 Pu75 A100 Py95 Py Subscripts = % occurrence Py = pyrimidine; Pu = purine; N = any nucleotide; nt = nucleotides BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 29 Splicing Introns from Eukaryotic mRNA Splicing ­ ­ ­ ­ ­ The discovery that eukaryotic genes were interrupted by intervening DNA The sequences (introns) iimmediately raised the question of how they would mmediately sequences be removed (spliced out). This is necessary if the mRNA is to be colinear with the protein it This colinear encodes. It is now known that RNA splicing involves the precise cleavage of It phosphodiester bonds at the exon-intron boundarie...
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This note was uploaded on 02/12/2014 for the course BIS 101 taught by Professor Simonchan during the Spring '08 term at UC Davis.

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