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Unformatted text preview: yotic Transcription
Eukaryotes use 3 different RNA
Eukaryotes polymerases (RNAPs) to transcribe
Eukaryotic promoters are similar to
prokaryotic promoters in that they
have three conserved regions needed
for proper promoter function:
q the Cap site,
q the TATA Box and
q the CAAT Box.
Eukaryotic mRNAs are typically
monocistronic, compared to the
polycistronic mRNAs found in
Eukaryotic mRNA is extensively
modified before it is used to make
protein. Processing includes capping,
tailing and splicing.
BIS101001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013
BIS101001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 4 Eukaryotes Use 3 Different RNAPs*
E.coli RNAP, ~480kDal, 4 subunits
plus RNAPI, ~470 kDal, 13 subunits, very
insensitive to fungal toxin, α-amanitin,
transcribes rRNA genes 17S, 25S and
5.8S (S values for yeast rRNA).
Ribosomal RNA is extensively
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This note was uploaded on 02/12/2014 for the course BIS 101 taught by Professor Simonchan during the Spring '08 term at UC Davis.
- Spring '08