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BIS101001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 20 Capping Reaction Summary BIS101001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013
BIS101001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 21 Tailing of Eukaryotic mRNA
Most eukaryotic mRNAs have a special "tail" added to the 3'
end. This tail is added by a special processing event that
recognizes sequence elements in the 3' untranslated region
(3' UTR) of eukaryotic mRNA and replaces the true 3' end of
the transcript with a tail consisting of 20-250 adenine
The signal in the 3' UTR of eukaryotic mRNA that triggers
tailing is the highly conserved sequence, AAUAAA (5'A98 A91
U100 A99 A983’).
A complex consisting of 4 proteins assembles at this sequence
to carry out the processing. The protein CPSF is a small
nuclear ribonucleoprotein or "snurp" that contains a small
RNA molecule (about 100...
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