11BIS1012013MicroRNALect11

Lrodriguez 2013

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Unformatted text preview: sion, R.L. Rodriguez 3 The RNA Interference (RNAi) Story Cosuppression Antisense Antisense RNA(aRNA) RNA(aRNA) Double-stranded RNA RNA interference (siRNA) (siRNA) (DsRNA = nuclear microRNA, long-hairpin (DsRNA dsRNA, or infectious dsRNA) dsRNA, Post-transcriptional silencing (knockdown) silencing BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 4 Cosupression and purple petals in petunias Extra dose of mRNA Extra for purple petal color for ­ Upon injection of the gene encoding mRNA responsible for purple Upon coloring in petunias, the flowers became variegated or white rather than deeper purple as was expected. What happened? deeper BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 5 RNA Interference (RNAi) 1998: Fire and Mello published a seminal paper on RNAi. Working with C. Fire elegans, they tested the hypothesis that the trigger for gene silencing was not single-stranded RNA (ssRNA) but double-stranded RNA (dsRNA). December 2, 2006, Andew Fire and Craig Mellow (shown below) receive Nobel Andew Prizes RNAi. Prizes BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 6 Antisense Mechanism BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 7 Biological function comes from proteins and RNAs Biological RNAs http://www.youtube.com/watch?v=cK-OGB1_ELE BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 8 Sources of dsRNA and their processing BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 9 MicroRNAs and Primary MicroRNAs MicroRNAs ­ Sho...
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This note was uploaded on 02/12/2014 for the course BIS 101 taught by Professor Simonchan during the Spring '08 term at UC Davis.

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