12BIS1012013TranslatLect12

n6 arrows indicate mutations

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Unformatted text preview: ial tRNAf-met and this tRNAf-met can not bind the ribosome without the help of IF2, f-met is not incorporated at other AUG codons on the mRNA. Likewise, normal tRNAmet can not be used to initiate translation because it is not tRNA recognized by IF2 and methionyl transformylase. BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 24 4 Differences between f-met-tRNA and met-tRNA ­ The following modifications permit the fMet-tRNA to be recognized by IF2 q (1) an unbound C-A base pair at the 5' end of the tRNA; (1) q (2) lack of an alkylated adenosine in the anticodon loop; (2) q (3) the presence an A in the T ΨC loop instead of a G and loop q (4) C-G bonding in the stem above the anticodon loop. -N6 (Arrows indicate mutations) BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 25 Requirements for Translation...
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