12BIS1012013TranslatLect12

Bis101001spring2013genesandgeneexpressionrlrodriguez

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Unformatted text preview: Rodriguez 15 Sucrose Gradients and “S” Values Sucrose “S” ­ ­ Macromolecular sedimentation coefficients (s) typically range from 10-11 to 10-13 seconds. In honor of Dr. Svedberg, the sedimentation coefficient 10 was changed to the Svedberg unit, or "S" value. For most Svedberg or macromolecules, S values range from 1 (10-11) to 100 (10-13). macromolecules, A typical velocity sedimentation ultracentrifugation experiment involves typical the use of a 5-20% sucrose gradient prepared in the centrifuge tube (see Figure below. BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 16 3-D Structure of Bacterial Ribosome 30S subunit 50S subunit Ribosome Ratcheting 70S subunit BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 17 3-D Structure of Bacterial Ribosome (ratcheting) Ribosome Ratcheting BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodr...
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