12BIS1012013TranslatLect12

Elongation the mrna is read on the ribosome in the 5

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Unformatted text preview: ez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 38 Bonding of the SD site to the 3’ end of 16S rRNA ­ Shown below is hydrogen bonding between the 3’’-end of the -end 16S rRNA (UCCUCCA) to the 5’-end of the (AGGAGGU) of the mRNA. This binding is part of the “pre-initiation” complex. BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 39 Bonding of the SD site to the 3’ end of 16S rRNA ­ ­ Shown below is a graph plotting –ΔG and +ΔG as a function Shown of nucleotide positions in and around the SD site. The closer of The the SG site is to the 3’end of the 16S RNA, the greater the -ΔG. BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 40 Three Stages of Translation ­ ­ ­ Initiation: In prokaryotes, protein synthesis begins with Nformyl-methionine (F-met)-tRNA bound to the 30S subunit in formyl-methionine a preinitiation complex. The tRNAf-met is positioned using the preinitiation ribosome binding site, so that the F-met amino...
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This note was uploaded on 02/12/2014 for the course BIS 101 taught by Professor Simonchan during the Spring '08 term at UC Davis.

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