12BIS1012013TranslatLect12

Lrodriguez 2013

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Unformatted text preview: Amino Acid # Synonymous Codons Leu, Ser, Arg Gly, Pro, Ala, Val, Thr Ile Phe, Tyr, Cys, His, Glu Gln, Asn, Asp, Lys Met, Trp Nonsense (ochre) Nonsense (opal) Nonsense (amber) Total Codons 6 4 3 18 20 3 2 0 18 2 UAA UGA UAG Total 64 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 10 Open Reading Frame (ORF) 5’-THEFATCATATETHEBIGRAT-3’ 0 5’-THE FAT CAT ATE THE BIG RAT-3’ +1 5’-T HEF ATC ATA TET HEB IGR AT-3’ Frameshifts +2 5’-TH EFA TCA TAT ETH EBI GRA T-3’ 0+1+2 5’­A T G C C T A T T G G G A T C T A T T A A T C C C­3 ’ 3’­T A C G G A T A A C C C T A G A T A A T T A G G G­5 ’ 2+1+0 6 possible ORFs for any segment of DNA BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 11 DNA can be read in 6 possible ways ­ The two strands of DNA can be read in the 5'' 3' direction, and on either strand there are The two 5 st three possible start point, at the...
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