12BIS1012013TranslatLect12

Lrodriguez 38 bonding of the sd site to the 3 end of

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Unformatted text preview: sequence are two additional Near sequences that play important roles in the initiation of translation. These are the SD site (Shine Dalgarno) and SD methionine codon, ATG (or AUG in the mRNA). methionine ATG The SD site, also referred to as the ribosome binding site The (RBS), is required for aligning the mRNA on the 30S subunit of the ribosome. This is accomplished by hydrogen bonding between the SD site on the mRNA and complementary sequences at the 3' end of the 16S rRNA in the 30S subunit. Once aligned, the methionine codon (AUG) is placed such Once that when bound by the aminoacyl methionine-charged tRNA, the methionine will be over the P site on the 50S subunit. the site BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodriguez 37 Shine Dalgarno Sites in Prokaryotic mRNAs ­ These SD sites in prokaryotes are conserved. Shown below These are 8 SD sites that have been used to generate the consensus sequence 5’-AGGAGG-3’ consensus BIS101­001, Spring 2013—Genes and Gene Expression, R.L. Rodrigu...
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This note was uploaded on 02/12/2014 for the course BIS 101 taught by Professor Simonchan during the Spring '08 term at UC Davis.

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