15BIS101F2013MutatLect15

Lrodriguez 2013

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: GAG ACA UGU Cys C G C G ATG UAC Tyr AA UU Val TC AG Glu Frameshift Mutation (deletion) Position DNA 123 5’ T T - 3’ A A RNA 5’ U U Amino acid Phe 4 5 6 7 8 9 10 11 12 TGT TAC G T T ACA ATG C UGU UAC G Val Thr Val Thr A U 13 14 15 GAG A C U G Leu Leu BIS101­001, Spring 2013—Genes and Gene Expression R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression R.L. Rodriguez T A C G 16 Effects of Various Point Mutations Wild Type Gene Sequence Position DNA 123 5’ T T T 3’ A A A RNA 5’ U U U Amino acid Phe 456 789 TGT TAC 10 11 12 GTT 13 14 15 GAG ACA UGU Cys C G C G ATG UAC Tyr AA UU Val TC AG Glu Frameshift Mutation (insertion) Position DNA 123 456 5’ T T T T T G T 789 TAC 10 11 12 GTT 3’ A A A A A C A A T G C A RNA 5’ U U U U U G U U A C G U Amino acid Phe Leu Leu Arg Leu Arg A U 13 14 15 GAG CT GA Non Non BIS101­001, Spring 2013—Genes and Gene Expression R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression R.L. Rodriguez C G 17 Intragenic Suppression of Point Mutations Wild Type Gene Sequence Position DNA 123 5’ T T T 3’ A A A RNA 5’ U U U Amino acid Phe 456 789 TGT TAC 10 11 12 GTT 13 14 15 GAG ACA UGU Cys C G C G ATG UAC Tyr AA UU Val TC AG Glu Frameshift Mutation (deletion) Position DNA 123 5’ T T - 3’ A A RNA 5’ U U Amino acid Phe 4 5 6 7 8 9 10 11 12 --T TAC G T T 13 14 15 GAG --A --U C G ATG UAC Tyr C G AA UU Val BIS101­001, Spring 2013—Genes and Gene Expression R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression R.L. Rodriguez TC AG Glu 18 Intragenic Suppression of Point Mutations Wild Type Gene Sequence Position DNA 123 5’ T T T 3’ A A A RNA 5’ U U U Amino acid Phe 456 789 TGT TAC 10 11 12 GTT 13 14 15 GAG ACA UGU Cys C G C G ATG UAC Tyr AA UU Val TC AG Glu Frameshift Mutation (insertion) Position DNA 123 5’ T T - 3’ A A RNA 5’ U U Amino acid Phe 456 789 TGTTTAC 10 11 12 GTT 13 14 15 GAG ACAAATG UGUUUAC Val Tyr Val C G C G AA UU Val BIS101­001, Spring 2013—Genes and Gene Expression R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression R.L. Rodriguez TC AG Glu Glu 19 Extragenic Suppression of Mutation As shown in the previous example, the affect of a mutation As can be covered up or “suppressed” by a second mutation. This second mutation can occur in the mutated gene (intragenic suppression) or outside the gene or in another suppression or gene (extragenic suppression). gene The most common type of extragenic suppressor is a mutant The tRNA in which a base substitution in the anticodon loop enables the tRNA to recognized one of the stop codons (UAA, UGA, and UAG). UAG amber mutation Gene A X tRNAtyr1 tRNAtyr2 X Anticodon mutation BIS101­001, Spring 2013—Genes and Gene Expression R.L. Rodriguez ©2013 BIS101­001, Spring 2013—Genes and Gene Expression R.L. Rodriguez 20 Wild type and Tyrosine Amber Suppressor tRNA Courtesy Stanley Maloy, SDSU Center for Applied and Experimental Genomics BIS101­001, Spring 2013—Ge...
View Full Document

Ask a homework question - tutors are online