How many hypoxia regulated genes in zebrafish embryos

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Unformatted text preview: ble gene in zebrafish embryos and larvae Low O2 Normal O2 96 hpf Maures and Duan, Endocrinology (2002) Questions 1.  How does hypoxia induce the IGFBP-1 gene expression in fish embryos? What other genes are up-regulated by hypoxia in a developing embryo?" 2. What is the functional significance of the elevated IGFBP-1 expression under hypoxia?" " Our working model of how hypoxia induces IGFBP-1 expression Hypoxia HIF ­1β (ARNT) β HIF ­1α α Normoxia degradation Prolylhydroxylases HAS X" β α p300 HRE igkp1a -1099/-1103! -1090/-1086" HRE = hypoxia response element There are many many hypoxia regulated genes! How many? Hypoxia regulated genes in zebrafish embryos Hyp_VS_WT (12hpf) P < 0.05, fold change (FC) > 2 5212 Up ­regulated genes 4518 Down ­regulated genes Hif ­1α & Hif ­3α vs. WT p<0.01, FC>2 603 52 87 Hif-3α Hif-1α 139 690 Zhang et al., manuscript under review� Questions 1.  How does hypoxia induce the IGFBP-1 gene expression in fish embryos? What other genes are up-regulated by hypoxia in a developing embryo?" 2. What is the functional significance of the " elevated IGFBP-1 expression under hypoxia?" IGFBP-1 is a metabolically controlled molecular switch ! that inhibits IGF actions under catabolic st...
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