10.3.05 - Announcements Enyzyme lab pre-quiz Bring p a4-a12...

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October 3, 2005 enzymes Structure: Globular protein that fold into complex 3D structures called confirmations Function: Regulate cellular functions by binding to substrate on its active site. They also reduce activation energies. E + S ES EP E + P E = enzyme S = substrate P = product What enzymes do… Orentation maintain a correct orientation. Substrate stabilizes transitional state by alterind the electron distribution of the substrate. Causes physical stress on the chemical bonds that need to be broken, lowering activation energy. Enzymes & Environment Every enzyme has an optimal pH, temperature. When they are out of suitable environment, they Ionization of Water M to M from decreases H Soooo H pH M OH H PureWater Ex M OH H K OH H O H w 12 10 7 14 2 10 .. .. 10 .. .. ] [ .... ] log[ 10 ] [ ] [ , : 10 1 ] ][ [ - - + - - + - - + - + + - = = = × = = + Buffer System Maintain a stable pH Silectively binds and releases H+ Two important buffers in biological systems Example: Phosphate Buffer
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  • Summer '07
  • GILBERT,C.
  • Enzymes, Enzyme, pH, substrate concentration, available enzymes lessons, Bring p. a4-a12

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