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25 182 055 125 080 6 0167 261 038 158 063 8 0125 333

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Unformatted text preview: 5 1.82 0.55 1.25 0.80 6 0.167 2.61 0.38 1.58 0.63 8 0.125 3.33 0.30 1.82 0.55 10 0.1 4.0 0.25 2.00 0.50 [Hint: concentration and rates were chosen for a simple relation and inversions are given, so you could skip plotting if you see the trend, but, explain your method and mark your answer (KM, vR MAX) and type of inhibition. Use back if needed.] Answer [PGF] 2.00 4.00 6.00 8.00 10.00 1/[PGF] VR 0.50 0.25 0.17 0.13 0.10 0.95 1.82 2.61 3.33 4.00 1/VR indol inhib. vR indol inhib,1/VR 1.05 0.77 1.30 0.55 1.25 0.80 0.38 1.58 0.63 0.30 1.82 0.55 0.25 2.00 0.50 intercept > 1/vRMAX = 0.05 ‐‐> vRMAX = 20 slope ‐‐> KM/vRMAX = 2 ‐‐> KM = 40 mM‐1 inhib, same idea ‐‐> vRMAX = 3.33, KM = 6.66 mM‐1 uncompetitive ‐‐> parallel L‐B plots...
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