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Unformatted text preview: What re two reasons why this may be useful or needed? 3. Give three examples (in some detail) of protocols/methods used to carry out directed mutagenesis of cloned genes/proteins. 4. Give four examples of posttranslational modifications of eukaryotic proteins, which may affect the function of the product and an example of why it can be a problem if they are not done or done incorrectly. 5. Explain the importance of each of these in your choice of expression vectors for expressing a cloned gene product in prokaryotic cells (what is the function of each and why are they important/necessary). a. promoter (strength/regulation) b. ribosome binding site c. transcriptional terminator d. origin of replication e. multiple cloning site f. selectable marker(s) g. vector copy number...
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This note was uploaded on 04/08/2008 for the course BIOL 4770 taught by Professor Benjiman during the Spring '08 term at North Texas.
- Spring '08