10.12.05 - October 12, 2005 Dark reactions Review of Liught...

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Dark reactions Review of Liught Reactiions In Out Light & Water O2 ATP NADPH Overview of Dark Rxn CO2 + 5C 6C 2 3C ----NADPH reduced---> 3C ---out goes sugar--> 5C Phase 1: Carbon Fixation CO2 + 5C,2P(RuPb) 6C Water splits the 6C to two 3C, 1P Phase 2 Each 3C,1P phosphorylated (ATP gived up a P to become ADP) and becomes 3C, 2P Each 3C,2P is then reduced by NADPH, which becomeS NADP+, to become two 3C, 1P 1 3C,1P makes a G3P sugar; the other 5 (you must tuen the cycle 3 times to make 1 glucose) go on for regeneration Phase 3: RuPb Regeneration 5 G3P three 5C Chromatography Glyceric acid Energy Cost of Dark Reaction dG = +114 kcal/mole 6CO2 + 18ATP + 12NADPH + 12 H2O C6H1206 + 18ADP + 12NADP+ + 6H+ 30% efficeient Rubisco The most common enzyme on the planet Bad cuz unselective enzyme o Main Rxn: CO2 + RuBP 2 3phosphoglycerate (80%) o Also Photorespiration: O2 + RuBP 3C + 2 3phosphoglycerate (20%) o The O2 rxn is bad cuz it uses up ATP but produces nothing. o
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10.12.05 - October 12, 2005 Dark reactions Review of Liught...

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