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ORC then brings in
additional proteins that
load the MCM complex.
MCM is a hexameric
ring that, similar to the
sliding clamp, encircles
DNA and can’t fall o .
J. Blow and T. Tanaka, EMBO Rep. 6:1028 ORC Cdc6 Cdt1
ORC M M
Cdc6 M Cdt1
M M Licensing Fig 2 | Components of the pre-replicative complex. The licensing of a single
replication origin is shown. ORC is first recruited to chromatin, and then
recruits Cdc6 and Cdt1. The ORC–Cdc6–Cdt1 complex licenses the origin by
loading numerous Mcm2–7 complexes onto it. T h e s e c a n b e gr o u p e d i n t o f o u r c a t e g o r i e s ( p r o t e o l y s i s , i n h i b i t o r y
p h osp h ory l a t i o n , n u c l e ar e x p ort a n d i n h i b i t i o n b y ge m i n i n), h p://en.wikipedia.org/wiki/Cell_cycle
Th e c h r o m o s o m e c y c l e
J.J. Blow & T.U. Tanaka 26 Th e c h r o m o s o m e c y c l e
J.J. Blow & T.U. Tanaka (When) Origin licensing is linked to the cell cycle
Licensing is coupled to
loading with cohesin, which
chromosomes together until
the daughter chromosomes
are yanked apart during...
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