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Unformatted text preview: and were documented using GeneSnap
gel doc system (SynGene, Cambridge, UK).
2.7. Scoring and analysis of RAPD data
DNA fragments showing polymorphism were scored using
a binary system of 0 (absent) and 1 (present). Genetic
relatedness among the 9 regenerants was estimated using
Jaccard’s (1908) coefficient of similarity. Cluster analysis
was performed using the unweighted pair group method
with arithmetic mean (UPGMA). The genetic similarity
and dendrogram were computed using the Numerical
Taxonomy and Multivariate Analysis System (NTSYS)-pc
software, version 2.11a (32).
3.1. Effect of gamma irradiation on regeneration of
shoots from in vitro buds
The present study was conducted to determine the effects
of irradiation on regeneration and morphological changes
of E. elatior buds. Gamma rays are mainly used to irradiate
multicellular layers because of the high penetration rate
into tissues. Irradiation of E. elatior buds with gamma rays
ranging from 10 to 140 Gy showed decreasing survival of
the explants with increasing radiation doses. The effects of
increasing doses of radiation on r...
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This document was uploaded on 02/23/2014.
- Spring '14