This preview shows page 1. Sign up to view the full content.
Unformatted text preview: ation.
They were cultured on MS medium supplemented with
13.32 µM of BAP under dark conditions for the 8 weeks.
The use of gamma ray extended the days until first shoot
emergence from 2 weeks for the untreated explant to
6–12 weeks for the irradiated explant. New multiple shoot
regeneration began with visible proliferation of buds from
the basal part of the explants. Differences were observed
after 8 weeks of irradiation with regard to the mean
number of shoots and mean number of leaves produced
Poor regeneration frequency was observed in the
postirradiation culture. The shoots showed slow growth
but were identical to the control treatment in terms of
morphology (Figure 2D). Gamma-ray irradiation did not
affect the ability to produce M1V2 and M1V3 shoots by
the surviving irradiated buds. When the irradiation dose
was more than 10 Gy, the new shoots derived from the
gamma-irradiated explants grew slower than the control.
A minimum of 12 weeks were needed for new shoots from
the irradiated explants (M1V0) to reach 1 cm in length,
View Full Document
This document was uploaded on 02/23/2014.
- Spring '14