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Unformatted text preview: gement of chimera and the biological effect of
The use of multicellular cultures for mutant induction
in vegetatively propagated crops has been suggested,
although reports have indicated the difficulties in obtaining
solid mutants (Gavidia and Perez-Bermudez, 1999).
For mutation breeding, the production of nonchimeric
mutants is highly desirable. Gamma radiation treatment
of multicellular tissues such as multiple buds often leads to
chimeras, depending on the occurrence of mutations in the
L1, L2, and L3 meristematic layers (Jain, 2010). Unstable
mericlinal chimeras automatically occur when plant
parts with multicellular layers are irradiated. Fortunately,
chimeras can be converted into solid mutants by repeated
multiplication (Yang and Schmidt, 1994). Therefore, it
is highly desirable to dissociate chimeras by subsequent
subcultures up to M1V3–M1V4 generations (Jain et al.,
1998; Mandal et al., 2000). This should be done to maintain
the stability of mutant traits and to ensure that the selected
mutant lines are solid mutants or stable periclinal chimeras.
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