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Unformatted text preview: .4. Evaluation of genetic variation with RAPD markers
The present study aimed to produce useful variants through
gamma irradiation and analyze the variation at a genetic level
using RAPD. Traditionally, the identification of variation
has been based on morphological characters. However,
E. elatior has a long flowering cycle (around 1–2 years to
flowering). Hence, morphological variation in flowers can
only be detected at a late developmental stage. Therefore,
early detection of genetic variability is a critical requirement 8 in an E. elatior breeding program. The development of PCRbased techniques such as RAPD has allowed analyses based
on DNA information (Martin et al., 2002). The PCR profile
amplified by RAPD markers successfully detected genetic
variations among the induced mutant clones of E. elatior.
The level of similarity was in all cases less than 90%. The
possible genotypic differences might have occurred based
on the sensitivity of the irradiated tissue to mutagenic
treatments (Ahloowalia, 1998)....
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- Spring '14