44 evaluation of genetic variation with rapd markers

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Unformatted text preview: .4. Evaluation of genetic variation with RAPD markers The present study aimed to produce useful variants through gamma irradiation and analyze the variation at a genetic level using RAPD. Traditionally, the identification of variation has been based on morphological characters. However, E. elatior has a long flowering cycle (around 1–2 years to flowering). Hence, morphological variation in flowers can only be detected at a late developmental stage. Therefore, early detection of genetic variability is a critical requirement 8 in an E. elatior breeding program. The development of PCRbased techniques such as RAPD has allowed analyses based on DNA information (Martin et al., 2002). The PCR profile amplified by RAPD markers successfully detected genetic variations among the induced mutant clones of E. elatior. The level of similarity was in all cases less than 90%. The possible genotypic differences might have occurred based on the sensitivity of the irradiated tissue to mutagenic treatments (Ahloowalia, 1998)....
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